Simple screening method for lipase for transesterification in organic solvent

被引：30

作者：

Furutani, T ^{[1
]}

Su, RH ^{[1
]}

Ooshima, H ^{[1
]}

Kato, J ^{[1
]}

机构：

[1] OSAKA CITY UNIV,FAC ENGN,DEPT BIOAPPL CHEM,SUMIYOSHI KU,OSAKA 558,JAPAN

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 12期

关键词：

lipase; substrate specificity; p-nitrophenyl esters; hydrolysis; transesterification; organic solvent;

D O I：

10.1016/0141-0229(95)00035-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated lipase-catalyzed hydrolysis in water and dioxane-water with a simple colorimetric method. We screened 24 lipases for the ability to hydrolyze p-nitrophenyl esters as chromogenic substrates. Their hydrolytic activities were varied by adding dioxane. Most of the lipases showed high activity in hydrolysis in water, but some showed activity in 50% dioxane-water several tens times higher than those in water. Moreover, several lipases with hydrolytic abilities in 50% dioxane-water also catalyzed the transesterification of p-nitrophenol using fatty acid vinyl esters. We found it possible that a useful lipase for transesterification can be selected by measuring the hydrolysis activity of p-nitrophenyl ester in 50% dioxane-water.

引用

页码：1067 / 1072

页数：6

共 16 条

[1] A BIOCATALYTIC APPROACH TO NONRACEMIC 2-METHYL-3-(2-THIOPHENE)-1-PROPANOLS AS CHIRAL BUILDING-BLOCKS FOR THE SYNTHESIS OF PYRIDINE ALKALOIDS
BRACHER, F
PAPKE, T
[J]. TETRAHEDRON-ASYMMETRY, 1994, 5 (09) : 1653 - 1656
[2] ESTERASE-ACTIVITY OF PURE CULTURES OF RUMEN BACTERIA AS EXPRESSED BY THE HYDROLYSIS OF PARA-NITROPHENYLPALMITATE
FAY, JP
JAKOBER, KD
CHENG, KJ
COSTERTON, JW
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1990, 36 (08) : 585 - 589
[3] Gatt S, 1981, Methods Enzymol, V72, P351
[4] DRASTIC SOLVENT EFFECT ON LIPASE-CATALYZED ENANTIOSELECTIVE HYDROLYSIS OF PROCHIRAL 1,4-DIHYDROPYRIDINES
HIROSE, Y
KARIYA, K
SASAKI, I
KURONO, Y
EBIIKE, H
ACHIWA, K
[J]. TETRAHEDRON LETTERS, 1992, 33 (47) : 7157 - 7160
[5] KAN N, 1992, 25TH P AUT M SCEJ, V103, P364
[6] RESOLUTION OF RACEMIC MIXTURES VIA LIPASE CATALYSIS IN ORGANIC-SOLVENTS
KIRCHNER, G
SCOLLAR, MP
KLIBANOV, AM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (24) : 7072 - 7076
[7] LIPASE-CATALYZED ASYMMETRIC HYDROLYSIS OF 3-PHENYLGLYCIDIC ACID ESTER, THE KEY INTERMEDIATE IN THE SYNTHESIS OF DILTIAZEM HYDROCHLORIDE
MATSUMAE, H
FURUI, M
SHIBATANI, T
[J]. JOURNAL OF FERMENTATION AND BIOENGINEERING, 1993, 75 (02): : 93 - 98
[8] MAURICH V, 1991, J CHROMATOGR, V556, P453
[9] LIPASE-CATALYZED RESOLUTION OF 5,5-DISUBSTITUTED HYDANTOINS
MIZUGUCHI, E
ACHIWA, K
WAKAMATSU, H
TERAO, Y
[J]. TETRAHEDRON-ASYMMETRY, 1994, 5 (08) : 1407 - 1410
[10] MIZUGUCHI E, 1993, TETRAHEDRON-ASYMMETR, V44, P2303

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