Activity of glucose oxidase entrapped in mesoporous gels

被引:45
作者
Han, KC
Wu, ZJ
Lee, J
Ahn, IS
Park, JW
Min, BR
Lee, KT
机构
[1] Yonsei Univ, Dept Chem Engn, Seoul 120749, South Korea
[2] Huaqiao Univ, Coll Mat Sci & Engn, Quanzhou 362011, Peoples R China
基金
新加坡国家研究基金会;
关键词
glucose; glucose oxidase; enzyme activity; immobilization; immobilized enzyme; mesoporous gel;
D O I
10.1016/j.bej.2004.09.011
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have immobilized a glucose oxidase (GOD) in mesoporous gels derived from tetraethoxysilane (TEOS) and organosilanes (i.e., methyltriethoxysilane (MTES), vinyltriethoxysilane (VTES), propyltriethoxysilane (PTES), and phenyltriethoxysilane (PhTES)) through a two-step sol-gel process without any templating agent. To find an optimal condition that maximizes the activity of the entrapped GOD, we investigate the effects of the organosilane precursor and the aging and drying temperature on the activity of the entrapped GOD. Our experimental results demonstrate that the activity of the entrapped GOD is highest when the gel is aged and dried at 4 degreesC (except for the PhTES/TEOS gels), while it is lowest when the gel is aged and dried at room temperature. We find that the use of organosilane does not significantly improve the activity of the entrapped GOD. These findings suggest a sol-gel route that can maximize the activity of the entrapped enzyme. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:161 / 166
页数:6
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