Distinct roles of P2X receptors in modulating glutamate release at different primary sensory synapses in rat spinal cord

被引:77
作者
Nakatsuka, T [1 ]
Tsuzuki, K [1 ]
Ling, JX [1 ]
Sonobe, H [1 ]
Gu, JGG [1 ]
机构
[1] Univ Florida, Coll Dent, Dept Oral Surg, Div Neurosci,McKnight Brain Inst, Gainesville, FL 32610 USA
关键词
D O I
10.1152/jn.01172.2002
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Using spinal cord slice preparations and patch-clamp recordings in lamina II and lamina V regions, we tested a hypothesis that P2X receptor subtypes differentially modulate glutamate release from primary afferent terminals innervating different sensory regions. We found that activation of P2X receptors by alpha,beta-methylene-ATP increased glutamate release onto >80% of DH neurons in both lamina regions. However, two distinct types of modulation, a transient and a long-lasting enhancement of glutamate release were observed. In lamina II recordings, >70% of the modulation was transient. In contrast, P2X receptor-mediated modulation was always long-lasting in lamina V. Pharmacologically, both transient and long-lasting types of modulation were blocked by 10 muM pyridxal-phospahte-6-azophenyl-2',4'-disulphonic acid tetrasodium, a broad-spectrum P2X receptor antagonist. Transient modulation was not observed in the presence of 1 muM trinitrophenyl-ATP (TNP-ATP), a subtype-selective P2X receptor antagonist, suggesting that homomeric P2X(3) receptors may be involved in the transient modulation in lamina II. The long-lasting modulation remained in the presence of 1 muM TNP-ATP. Selective removal of P2X(3)-expressing afferent terminals by the targeting toxin saporin-conjugated isolectin B4 or surgical removal of superficial DH did not affect P2X receptor-mediated long-lasting modulation in lamina V. Taken together, these results suggest that P2X receptor subtypes play distinct roles in sensory processing in functionally different sensory regions.
引用
收藏
页码:3243 / 3252
页数:10
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