Directed evolution: the 'rational' basis for 'irrational' design

被引：93

作者：

Tobin, MB ^{[1
]}

Gustafsson, C ^{[1
]}

Huisman, GW ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(00)00109-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple and dispersed substitutions that act synergistically to improve enzyme properties and function. The benefits of such multiple changes are often not predictable from a priori structural knowledge. Furthermore, alternative solutions to gaining functional change can be obtained.

引用

页码：421 / 427

页数：7

共 59 条

[1] RETRACTED: Directed evolution of new catalytic activity using the α/β-barrel scaffold (Retracted article. See vol 417, pg 468, 2002) [J].