Directed evolution: the 'rational' basis for 'irrational' design

被引：93

作者：

Tobin, MB ^{[1
]}

Gustafsson, C ^{[1
]}

Huisman, GW ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(00)00109-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple and dispersed substitutions that act synergistically to improve enzyme properties and function. The benefits of such multiple changes are often not predictable from a priori structural knowledge. Furthermore, alternative solutions to gaining functional change can be obtained.

引用

收藏

页码：421 / 427

页数：7

相关论文

共 59 条

[21] Combinatorial protein design by in vitro recombination [J].

Giver, L ;

Arnold, FH .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (03) :335-338

[22] EXHAUSTIVE MATCHING OF THE ENTIRE PROTEIN-SEQUENCE DATABASE [J].

GONNET, GH ;

COHEN, MA ;

BENNER, SA .

SCIENCE, 1992, 256 (5062) :1443-1445

[23] Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling [J].

Hansson, LO ;

Bolton-Grob, R ;

Massoud, T ;

Mannervik, B .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 287 (02) :265-276

[24] Artificial evolution by DNA shuffling [J].

Harayama, S .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (02) :76-82

[25] Directed evolution of an esterase from Psueudomonas fluorescens.: Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay [J].

Henke, E ;

Bornscheuer, UT .

BIOLOGICAL CHEMISTRY, 1999, 380 (7-8) :1029-1033

[26] Directed evolution of thermostable kanamycin-resistance gene:: A convenient selection marker for Thermus thermophilus [J].

Hoseki, J ;

Yano, T ;

Koyama, Y ;

Kuramitsu, S ;

Kagamiyama, H .

JOURNAL OF BIOCHEMISTRY, 1999, 126 (05) :951-956

[27] Microbial lipases form versatile tools for biotechnology [J].

Jaeger, KE ;

Reetz, MT .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (09) :396-403

[28] Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation [J].

Joo, H ;

Lin, ZL ;

Arnold, FH .

NATURE, 1999, 399 (6737) :670-673

[29] Novel family shuffling methods for the in vitro evolution of enzymes [J].

Kikuchi, M ;

Ohnishi, K ;

Harayama, S .

GENE, 1999, 236 (01) :159-167

[30] An effective family shuffling method using single-stranded DNA [J].

Kikuchi, M ;

Ohnishi, K ;

Harayama, S .

GENE, 2000, 243 (1-2) :133-137

← 1 2 3 4 5 6 →