Binding of symmetrical cyanine dyes into the DNA minor groove

被引:36
作者
Mikheikin, AL [1 ]
Zhuze, AL
Zasedatelev, AS
机构
[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117984, Russia
[2] Moscow Inst Phys & Technol, Dolgoprudnyi 141700, Russia
关键词
D O I
10.1080/07391102.2000.10506647
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Optical methods, such as fluorescence, circular dichroism and linear flow dichroism, were used to study the binding to DNA of four symmetrical cyanine dyes, each consisting of two identical quinoline, benzthiazole, indole, or benzoxazole fragments connected by a trimethine bridge. The ligands were shown to form a monomer type complex into the DNA minor groove. The complex of quinoline-containing ligand with calf thymus DNA appeared to be the most resistant to ionic strength, and it did not dissociate completely even in 1 M NaCl. Binding of cyanine dyes to DNA could also be characterized by possibility to form ligand dimers into the DNA minor groove, by slight preference of binding to AT pairs, as well as by possible intercalation between base pairs of poly(dG) poly(dC). The correlation found between the binding constants to DNA and the extent of cyanine dyes hydrophobicity estimated as the n-octanol/water partition coefficient is indicative of a significant role of hydrophobic interactions for the ligand binding into the DNA minor groove.
引用
收藏
页码:59 / 72
页数:14
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