Preliminary study on hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilized on Sepharose

被引：9

作者：

Diogo, MM

Cabral, JMS

Queiroz, JA ^{[1
]}

机构：

[1] Univ Beira Interior, Dept Quim, P-6200 Covilha, Portugal

[2] Inst Super Tecn, Engn Bioquim Lab, P-1000 Lisbon, Portugal

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 796卷 / 01期

关键词：

Chromobacterium viscosum; mobile phase composition; lipase; enzymes;

D O I：

10.1016/S0021-9673(97)01059-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The purification of Chromobacterium viscosum lipase was performed using a polypropylene glycol-Sepharose gel. The influence of the mobile phase composition on the chromatographic behaviour of Chromobacterium viscosum lipase was studied and it was found that the retention of lipase depends on the salt used and increased with ionic strength. Using 20% (w/v) ammonium sulphate in the eluent, a total retention of lipase on the column was obtained. (C) 1998 Elsevier Science B.V.

引用

页码：177 / 180

页数：4

共 12 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] PROTEIN ADSORBENTS INTENDED FOR USE IN AQUEOUS 2-PHASE SYSTEMS [J].