Denaturing gradient gel electrophoresis (DGGE) screening of clones prior to sequencing

被引：5

作者：

Middleton, SA

Anzenberger, G

Knapp, LA

机构：

[1] Univ Cambridge, Dept Biol Anthropol, Cambridge CB2 3DZ, England

[2] Univ Zurich, Anthropol Inst & Museum, CH-8057 Zurich, Switzerland

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期

关键词：

clones; DGGE; screening; sequencing;

D O I：

10.1111/j.1471-8286.2004.00799.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whilst cloning and sequencing techniques are used ubiquitously for the identification of novel DNA sequences, the necessity to determine a consensus sequence means that this can be both labour-intensive and costly. Here we describe a rapid and cost effective method of using denaturing gradient gel electrophoresis (DGGE) for the analysis of large numbers of clones prior to sequencing. This procedure allows for the selection of specific clones, eliminates the need to sequence multiple copies of the same clone, and reduces the likelihood of sequencing recombinant PCR product.

引用

收藏

页码：776 / 778

页数：3

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