Purification and identification of a growth-stimulating peptide for Bifidobacterium bifidum from natural rubber serum powder

被引:17
作者
Etoh, S [1 ]
Asamura, K [1 ]
Obu, A [1 ]
Sonomoto, K [1 ]
Ishizaki, A [1 ]
机构
[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Div Biosci & Biotechnol, Lab Microbial Sci & Technol,Higashi Ku, Fukuoka 8128581, Japan
关键词
natural rubber serum powder; Bifidobacterium; growth-stimulating peptide; purification;
D O I
10.1271/bbb.64.2083
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Natural rubber serum powder, which is a by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254. The retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on B-12 assay medium with ammonium sulfate. One of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic pretreatment column, and multistage reversed-phase HPLC. An increase of 53-fold in the specific activity, and a recovery of 1.3% were obtained. The amino acid composition and N-terminal sequence analysis of this growth stimulator provided the structure of Ala-Thr-Pro-Glu-Lys-Glu-Glu-Pro-Thr-Ala. The molecular mass was 1075 by MALDI-TOF MS analysis. These results showed that this growth stimulator was a decapeptide with the sequence shown above. This is the first report that clarified the structure of an active peptide for the growth of Bifidobacterium.
引用
收藏
页码:2083 / 2088
页数:6
相关论文
共 22 条
[1]   BIFIDUS GROWTH-PROMOTING ACTIVITY OF A GLYCOMACROPEPTIDE DERIVED FROM HUMAN K-CASEIN [J].
AZUMA, N ;
YAMAUCHI, K ;
MITSUOKA, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (08) :2159-2162
[2]  
CHENG C-C, 1985, Japanese Journal of Zootechnical Science, V56, P484
[3]  
CHENG C-C, 1985, Japanese Journal of Zootechnical Science, V56, P257
[4]  
DESJARDINS ML, 1991, MILCHWISSENSCHAFT, V46, P11
[5]  
ELSODA M, 1992, MILCHWISSENSCHAFT, V47, P87
[6]   Complementary effects of bifidogenic growth stimulators and ammonium sulfate in natural rubber serum powder on Bifidobacterium bifidum [J].
Etoh, S ;
Sonomoto, K ;
Ishizaki, A .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (04) :627-631
[7]   Modification of the intestinal microflora using probiotics and prebiotics [J].
Fuller, R ;
Gibson, GR .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1997, 32 :28-31
[8]   Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk hydrolyzates [J].
Gomes, AMP ;
Malcata, FX ;
Klaver, FAM .
JOURNAL OF DAIRY SCIENCE, 1998, 81 (11) :2817-2825
[9]   Use of small ruminants' milk supplemented with available nitrogen as growth media for Bifidobacterium lactis and Lactobacillus acidophilus [J].
Gomes, AMP ;
Malcata, FX .
JOURNAL OF APPLIED MICROBIOLOGY, 1998, 85 (05) :839-848
[10]   PHYTOCHELATINS - THE PRINCIPAL HEAVY-METAL COMPLEXING PEPTIDES OF HIGHER-PLANTS [J].
GRILL, E ;
WINNACKER, EL ;
ZENK, MH .
SCIENCE, 1985, 230 (4726) :674-676