The use of RNAi-based screens to identify host proteins involved in viral replication

被引：54

作者：

Hirsch, Alec J. ^{[1
]}

机构：

[1] Oregon Hlth & Sci Univ, Vaccine & Gene Therapy Inst, Beaverton, OR 97006 USA

来源：

FUTURE MICROBIOLOGY | 2010年 / 5卷 / 02期

基金：

美国国家卫生研究院;

关键词：

flavivirus; HIV; influenza; screen; siRNA; viral host factor; virus; C VIRUS-REPLICATION; STATISTICAL-METHODS; CELLULAR COFACTORS; TARGET RNA; INTERFERENCE; IDENTIFICATION; CELLS; INFECTION;

D O I：

10.2217/FMB.09.121

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins known to be encoded by the human genome. These libraries have been used to identify the host proteins required for the replication of several clinically important viruses, including HIV, flaviviruses and influenza. This review summarizes the methods used in RNAi-based screening for host factors involved in virus replication, and discusses published examples of such screens.

引用

页码：303 / 311

页数：9

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