Binding site of Zn2+ in ATP:N1 at low pH and N7 at high pH as evidenced by 1H-15N NMR HMBC experiments

被引：15

作者：

Du, F

Mao, XA

机构：

[1] Chinese Acad Sci, Wuhan Inst Phys & Math, Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Peoples R China

[2] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2000年 / 56卷 / 12期

基金：

中国国家自然科学基金;

关键词：

zinc; abundant adenosine-5 '-triphosphate; binding site; pH; heteronuclear multiple bond correlation;

D O I：

10.1016/S1386-1425(00)00286-9

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Nuclear magnetic resonance (NMR) H-1-N-15 heteronuclear multiple bond correlation (HMBC) experiments on natural abundant adenosine-5'-triphosphate (ATP) showed that zinc(II) induced large chemical shifts (> 10 ppm) for N1 at lower pH (2-5) while for N7 at higher pH (5-7), suggesting that the binding site of Zn2+ in the purine base of ATP is pH dependent. The size effect of zinc(II) in coordination is also discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：2391 / 2395

页数：5

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