A high-fat, ketogenic diet induces a unique metabolic state in mice

被引:334
作者
Kennedy, Adam R.
Pissios, Pavlos
Otu, Hasan
Xue, Bingzhong
Asakura, Kenji
Furukawa, Noburu
Marino, Frank E.
Liu, Fen-Fen
Kahn, Barbara B.
Libermann, Towia A.
Maratos-Flier, Eleftheria
机构
[1] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Endocrinol,Dept Med, Boston, MA 02215 USA
[2] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Genome Ctr,Dept Med, Boston, MA 02215 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 2007年 / 292卷 / 06期
关键词
liver; gene expression;
D O I
10.1152/ajpendo.00717.2006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Ketogenic diets have been used as an approach to weight loss on the basis of the theoretical advantage of a low- carbohydrate, high- fat diet. To evaluate the physiological and metabolic effects of such diets on weight we studied mice consuming a very- low- carbohydrate, ketogenic diet ( KD). This diet had profound effects on energy balance and gene expression. C57BL/ 6 mice animals were fed one of four diets: KD; a commonly used obesogenic high- fat, high- sucrose diet ( HF); 66% caloric restriction ( CR); and control chow ( C). Mice on KD ate the same calories as mice on C and HF, but weight dropped and stabilized at 85% initial weight, similar to CR. This was consistent with increased energy expenditure seen in animals fed KD vs. those on C and CR. Microarray analysis of liver showed a unique pattern of gene expression in KD, with increased expression of genes in fatty acid oxidation pathways and reduction in lipid synthesis pathways. Animals made obese on HF and transitioned to KD lost all excess body weight, improved glucose tolerance, and increased energy expenditure. Analysis of key genes showed similar changes as those seen in lean animals placed directly on KD. Additionally, AMP kinase activity was increased, with a corresponding decrease in ACC activity. These data indicate that KD induces a unique metabolic state congruous with weight loss.
引用
收藏
页码:E1724 / E1739
页数:16
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