Regulation of 25-hydroxyvitamin D3 1α-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3

被引:111
作者
Brenza, HL [1 ]
DeLuca, HF [1 ]
机构
[1] Univ Wisconsin, Dept Biochem, Coll Agr & Life Sci, Madison, WI 53706 USA
关键词
1; alpha-hydroxylase; parathyroid hormone; vitamin D; kidney; thyroparathyroidectomy;
D O I
10.1006/abbi.2000.1970
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The conversion of 25-hydroxyvitamin D-3 to 1,25-dihydroxyvitamin D-3 (1,25-(OH)(2)D-3) takes place mainly in the kidney and is catalyzed by the enzyme 1 alpha-hydroxylase, Parathyroid hormone (PTH) and 1,25-(OH)(2)D-3 are well-known regulators of this tightly controlled step, but the mechanisms by which they modulate 1 alpha-hydroxylase activity have not been fully delineated. Northern analysis showed PTH and forskolin rapidly and transiently increase 1 alpha-hydroxylase expression in AOK-B50 cells and HKC-8 cells. Actinomycin D treatment blocks the increase, but cycloheximide does not. No decrease of 1 alpha-hydroxylase transcript by 1,25-(OH)(2)D-3 was observed in either cell line, although 24-hydroxylase levels were strongly induced by 1,25-(OH)(2)D-3 treatment. 1,25-(OH)(2)D-3 suppressed the 1 alpha-hydroxylase transcript in vivo both in the presence and absence of exogenously supplied PTH, These results suggest that the stimulatory action of PTH is directly on the 1 alpha-hydroxylase gene, while the repressive action of 1,25-(OH)(2)D-3 does not involve the parathyroid gland but is nevertheless indirect. (C) 2000 Academic Press.
引用
收藏
页码:143 / 152
页数:10
相关论文
共 45 条
[1]   EXPRESSION CLONING OF A COMMON RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID HORMONE-RELATED PEPTIDE FROM RAT OSTEOBLAST-LIKE CELLS - A SINGLE RECEPTOR STIMULATES INTRACELLULAR ACCUMULATION OF BOTH CAMP AND INOSITOL TRISPHOSPHATES AND INCREASES INTRACELLULAR FREE CALCIUM [J].
ABOUSAMRA, AB ;
JUPPNER, H ;
FORCE, T ;
FREEMAN, MW ;
KONG, XF ;
SCHIPANI, E ;
URENA, P ;
RICHARDS, J ;
BONVENTRE, JV ;
POTTS, JT ;
KRONENBERG, HM ;
SEGRE, GV .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :2732-2736
[2]  
ARMBRECHT HJ, 1984, AM J PHYSIOL, V246, pE102
[3]   Constitutive expression of 25-hydroxyvitamin D3-1α-hydroxylase in a transformed human proximal tubule cell line:: Evidence for direct regulation of vitamin D metabolism by calcium [J].
Bland, R ;
Walker, EA ;
Hughes, SV ;
Stewart, PM ;
Hewison, N .
ENDOCRINOLOGY, 1999, 140 (05) :2027-2034
[4]   Tissue-specific expression of promoter regions of the alpha 1(VI) collagen gene in cell cultures and transgenic mice [J].
Braghetta, P ;
Vitale, P ;
Piccolo, S ;
Bonaldo, P ;
Fabbro, C ;
Girotto, D ;
Volpin, D ;
Bressan, GM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 247 (01) :200-208
[5]   Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α-hydroxylase gene promoter [J].
Brenza, HL ;
Kimmel-Jehan, C ;
Jehan, F ;
Shinki, T ;
Wakino, S ;
Anazawa, H ;
Suda, T ;
DeLuca, HF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (04) :1387-1391
[6]   CLONED, STABLY EXPRESSED PARATHYROID-HORMONE (PTH)/PTH-RELATED PEPTIDE RECEPTORS ACTIVATE MULTIPLE MESSENGER SIGNALS AND BIOLOGICAL RESPONSES IN LLC-PK1 KIDNEY-CELLS [J].
BRINGHURST, FR ;
JUPPNER, H ;
GUO, J ;
URENA, P ;
POTTS, JT ;
KRONENBERG, HM ;
ABOUSAMRA, AB ;
SEGRE, GV .
ENDOCRINOLOGY, 1993, 132 (05) :2090-2098
[7]   CLONING OF THE HUMAN 1-ALPHA,25-DIHYDROXYVITAMIN-D-3 24-HYDROXYLASE GENE PROMOTER AND IDENTIFICATION OF 2 VITAMIN-D-RESPONSIVE ELEMENTS [J].
CHEN, KS ;
DELUCA, HF .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1995, 1263 (01) :1-9
[8]  
CHEN PS, 1956, ANAL CHEM, V28, P1956
[9]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[10]   LOCAL-ACTION OF PHOSPHATE-DEPLETION AND INSULIN-LIKE GROWTH-FACTOR-1 ON IN-VITRO PRODUCTION OF 1,25-DIHYDROXYVITAMIN-D BY CULTURED MAMMALIAN KIDNEY-CELLS [J].
CONDAMINE, L ;
VZTOVSNIK, F ;
FRIEDLANDER, G ;
MENAA, C ;
GARABEDIAN, M .
JOURNAL OF CLINICAL INVESTIGATION, 1994, 94 (04) :1673-1679