Targeting neural circuitry in zebrafish using GAL4 enhancer trapping

被引：279

作者：

Scott, Ethan K.

Mason, Lindsay

Arrenberg, Aristides B.

Ziv, Limor

Gosse, Nathan J.

Xiao, Tong

Chi, Neil C.

Asakawa, Kazuhide

Kawakami, Koichi

Baier, Herwig ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA

[3] Grad Univ Adv Studies, SOKENDAI, Natl Inst Genet, Div Mol & Dev Biol, Mishima, Shizuoka 4118540, Japan

[4] Grad Univ Adv Studies, SOKENDAI, Dept Genet, Mishima, Shizuoka 4118540, Japan

来源：

NATURE METHODS | 2007年 / 4卷 / 04期

关键词：

GENE-EXPRESSION; IN-VIVO; TRANSPOSON;

D O I：

10.1038/NMETH1033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.

引用

页码：323 / 326

页数：4

共 12 条

[1] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein [J].