An ultrahighly sensitive and selective electrochemical DNA sensor via nicking endonuclease assisted current change amplification

被引：57

作者：

Chen, Jinghua ^{[1
]}

Zhang, Jing ^{[1
]}

Li, Juan ^{[1
]}

Fu, Fengfu ^{[1
]}

Yang, Huang-Hao ^{[1
]}

Chen, Guonan ^{[1
]}

机构：

[1] Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety,Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350002, Fujian, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 32期

关键词：

SEQUENCE-SPECIFIC DETECTION; ROLLING-CIRCLE AMPLIFICATION; SIGNAL AMPLIFICATION; FEMTOMOLAR DNA; HYBRIDIZATION DETECTION; NUCLEIC-ACIDS; BLOOD-SERUM; ELECTRODE; PROBE; QUANTIFICATION;

D O I：

10.1039/c0cc00748j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Based on the "nicking endonuclease assisted'' detection strategy, a novel electrochemical biosensor for the sequence-specific detection of DNA with ultrahigh sensitivity and selectivity is developed. By employing the above strategy, this DNA biosensor can detect as low as 0.068 fM target DNA and exhibits high discrimination ability even against a single-base mismatch.

引用

页码：5939 / 5941

页数：3

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