Monitoring the Activity of 2-Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde

被引：50

作者：

Hopkinson, Richard J. ^{[1
]}

Hamed, Refaat B. ^{[1
]}

Rose, Nathan R. ^{[1
]}

Claridge, Timothy D. W. ^{[1
]}

Schofield, Christopher J. ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 04期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

enzyme catalysis; formaldehyde; histone demethylases; NMR spectroscopy; transcriptional regulation; PERFORMANCE LIQUID-CHROMATOGRAPHY; SPECIFICITY; JMJD2A;

D O I：

10.1002/cbic.200900713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA.

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收藏

页码：506 / 510

页数：5

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