GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3

被引:80
作者
Tanida, I
Komatsu, M
Ueno, T
Kominami, E [1 ]
机构
[1] Juntendo Univ, Sch Med, Dept Biochem, Tokyo 1138421, Japan
[2] Tokyo Metropolitan Inst Med Sci, Dept Mol Oncol, Tokyo 1138613, Japan
关键词
Apg8/Aut7; LC3; GABARAP; GATE-16; ubiquitin-like modification; Apg7; Apg3; autophagy; lipidation;
D O I
10.1016/S0006-291X(02)02907-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
GATE-16, GABARAR and LC3 are three mammalian counterparts of yeast Apg8p/Aut7p. Here. we show that GATE-16 and GABARAP are authentic modifiers, as is the case of LC3 modification. The C-terminal Phet(117) of proGATE-16 and the C-terminal Leu(117) of proGABARAP are post-translationally cleaved to expose an essential Gly(116) within GATE-16 and GABARAP, with the products designated GATE-16-I and GABARAP-I. respectively. The Gly(116) within GATE-16 and GABARAP are essential for further formation of the intermediates between them and Apg7p(C572S) and Apg3p(C264S). When Apg7p and Apg3p are expressed. GATE-16-I and GABARAP-I are modified to a secondary ubiquitin-like modified form, GATE-16-II and GABARAP-II, respectively. GATE-16-I and GABARAP-I, but not LC3-I, localize to membrane compartments before their modification. These results indicate that GATE-16 and GABARAP are authentic modifiers, but that they have different biochemical characteristics from those of LC3. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:637 / 644
页数:8
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