Gramicidin channel kinetics under tension

被引：108

作者：

Goulian, M

Mesquita, ON

Fygenson, DK

Nielsen, C

Andersen, OS

Libchaber, A

机构：

[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA

[2] NEC Res Inst, Princeton, NJ 08540 USA

[3] Cornell Univ, Coll Med, Dept Physiol & Biophys, New York, NY 10021 USA

来源：

BIOPHYSICAL JOURNAL | 1998年 / 74卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(98)77790-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have measured the effect of tension on dimerization kinetics of the channel-forming peptide gramicidin A. By aspirating large unilamellar vesicles into a micropipette electrode, we are able to simultaneously monitor membrane tension and electrical activity. We find that the dimer formation rate increases by a factor of 5 as tension ranges from 0 to 4 dyn/cm. The dimer lifetime also increases with tension. This behavior is well described by a phenomenological model of membrane elasticity in which tension modulates the mismatch in thickness between the gramicidin dimer and membrane.

引用

收藏

页码：328 / 337

页数：10

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