Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells

被引：161

作者：

Li, G

Kim, DH

Kim, TD

Park, BJ

Park, HD

Park, JI

Na, MK

Kim, HC

Hong, ND

Lim, K

Hwang, BD

Yoon, WH

机构：

[1] Chungnam Natl Univ & Hosp, Canc Res Inst, Dept Surg, Coll Med, Taejon 301721, South Korea

[2] Chungnam Natl Univ, Coll Med, Dept Biochem, Taejon 301747, South Korea

[3] Chungnam Natl Univ, Canc Res Inst, Taejon 301721, South Korea

[4] Hankook Sin Yak Pharm Co, Ja Kwang Res Inst, Nonsan 320854, South Korea

来源：

CANCER LETTERS | 2004年 / 216卷 / 02期

关键词：

PL; apoptosis; antitumor effect; G2/M phase arrest;

D O I：

10.1016/j.canlet.2004.07.014

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The cytotoxic mechanism of protein-bound polysaccharide isolated from Phellinus linteus (PL, Mesimao(R)) has been investigated. PL inhibited the proliferation and colony formation of SW480 human colon cancer cells. Flow cytometry analysis showed that PL increased the populations of both apoptotic sub-G(1) and G(2)/M phase. The result obtained from TUNEL assay corroborated apoptosis which was shown in flow cytometry. Western blot analysis suggested that PL-induced apoptosis and growth inhibition were associated with decrease in Bcl-2, increase of the release of cytochrome c, and reduced expression of cyclin B1. These results suggest that PL has a direct antitumor effect through apoptosis and cell cycle blockade in certain cancer cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：175 / 181

页数：7

共 16 条

[1] Dysferlin in a hyperCKaemic patient with caveolin 3 mutation and in C2C12 cells after p38 MAP kinase inhibition [J].