Biofunctionalization of fluorescent rare-earth-doped lanthanum phosphate colloidal nanoparticles

被引：323

作者：

Meiser, F ^{[1
]}

Cortez, C ^{[1
]}

Caruso, F ^{[1
]}

机构：

[1] Univ Melbourne, Ctr Nanosci & Nanotechnol, Dept Chem & Biomol Engn, Melbourne, Vic 3010, Australia

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 44期

关键词：

biolabeling; colloids; lanthanides; luminescence; nanoparticles;

D O I：

10.1002/anie.200460856

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Approximately eight avidin molecules are conjugated to each Ce/Tb-doped lanthanum phosphate nanoparticle (NP) through the formation of amide bonds (see scheme). This biofunctionalization process comprises modification of the LaPO4 NPs with 6-aminohexanoic acid (AHA) to confer colloidal stability, activation of the AHA carboxy groups by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide, and avidin conjugation.

引用

页码：5954 / 5957

页数：4

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