Characterization of the RNA content of chromatin

被引：140

作者：

Mondal, Tanmoy ^{[2
]}

Rasmussen, Markus ^{[1
]}

Pandey, Gaurav Kumar ^{[2
]}

Isaksson, Anders ^{[1
]}

Kanduri, Chandrasekhar ^{[2
]}

机构：

[1] Uppsala Univ, Dept Med Sci Canc Pharmacol & Informat, S-75185 Uppsala, Sweden

[2] Uppsala Univ, Dept Genet & Pathol, S-75185 Uppsala, Sweden

来源：

GENOME RESEARCH | 2010年 / 20卷 / 07期

基金：

英国医学研究理事会;

关键词：

LONG NONCODING RNAS; TRANSCRIPTION; CONTRIBUTES; EXPRESSION; COMPONENT; PROTEIN; GENOME;

D O I：

10.1101/gr.103473.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Noncoding RNA (ncRNA) constitutes a significant portion of the mammalian transcriptome. Emerging evidence suggests that it regulates gene expression in cis or trans by modulating the chromatin structure. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harboring CARs. The intronic and intergenic CARs show significant conservation across 44 species of placental mammals. Functional characterization of one of the intergenic CARs, Intergenic10, revealed that it regulates gene expression of neighboring genes through modulating the chromatin structure in cis. Our data suggest that ncRNA is an integral component of chromatin and that it may regulate various biological functions through fine-tuning of the chromatin architecture.

引用

页码：899 / 907

页数：9

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