Persistence of vancomycin-resistant enterococci in New Zealand broilers after discontinuation of avoparcin use

被引:49
作者
Manson, JM [1 ]
Smith, JMB [1 ]
Cook, GM [1 ]
机构
[1] Univ Otago, Otago Sch Med Sci, Dept Microbiol, Dunedin, New Zealand
关键词
D O I
10.1128/AEM.70.10.5764-5768.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Large amounts of tylosin, zinc-bacitracin, and avilamycin are currently used as prophylactics in New Zealand broiler production. Avoparcin was also used from 1977 to 2000. A total of 382 enterococci were isolated from 213 fecal samples (147 individual poultry farms) using enrichment broths plated on m-Enterococcus agar lacking antimicrobials. These isolates were then examined to determine the prevalence of antimicrobial resistance. Of the 382 isolates, 5.8% (22 isolates) were resistant to vancomycin, and 64.7% were resistant to erythromycin. The bacitracin MIC was greater than or equal to256 mug/ml for 98.7% of isolates, and the avilamycin MIC was greater than or equal to8 mug/ml for 14.9% of isolates. No resistance to ampicillin or gentamicin was detected. Of the 22 vancomycin-resistant enterococci (VRE) isolates, 18 (81.8%) were Enterococcus faecalis, 3 were Enterococcus faecium, and I was Enterococcus durans. However, when the 213 fecal enrichment broths were plated on m-Enterococcus agar containing vancomycin, 86 VRE were recovered; 66% of these isolates were E. faecium and the remainder were E. faecalis. Vancomycin-resistant E. faecium isolates were found to have heterogenous pulsed-field gel electrophoresis (PFGE) patterns of SmaI-digested DNA, whereas the PFGE patterns of vancomycin-resistant E. faecalis isolates were identical or closely related, suggesting that this VRE clone is widespread throughout New Zealand. These data demonstrate that vancomycin-resistant E. faecalis persists in the absence and presence of vancomycin-selective pressure, thus explaining the dominance of this VRE clone even in the absence of avoparcin.
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页码:5764 / 5768
页数:5
相关论文
共 27 条
[1]   Associations between the use of antimicrobial agents for growth promotion and the occurrence of resistance among Enterococcus faecium from broilers and pigs in Denmark, Finland, and Norway [J].
Aarestrup, FM ;
Kruse, H ;
Tast, E ;
Hammerum, AM ;
Jensen, LB .
MICROBIAL DRUG RESISTANCE, 2000, 6 (01) :63-70
[2]   Incidence of high-level evernimicin resistance in Enterococcus faecium among food animals and humans [J].
Aarestrup, FM ;
McNicholas, PM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :3088-3090
[3]   Glycopeptide susceptibility among Danish Enterococcus faecium and Enterococcus faecalis isolates of animal and human origin and PCR identification of genes within the vanA cluster [J].
Aarestrup, FM ;
Ahrens, P ;
Madsen, M ;
Pallesen, LV ;
Poulsen, RL ;
Westh, H .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (08) :1938-1940
[4]   Effect of abolishment of the use of antimicrobial agents for growth promotion on occurrence of antimicrobial resistance in fecal enterococci from food animals in Denmark [J].
Aarestrup, FM ;
Seyfarth, AM ;
Emborg, HD ;
Pedersen, K ;
Hendriksen, RS ;
Bager, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (07) :2054-2059
[5]   Glycopeptide resistance in Enterococcus faecium from broilers and pigs following discontinued use of avoparcin [J].
Bager, F ;
Aarestrup, FM ;
Madsen, M ;
Wegener, HC .
MICROBIAL DRUG RESISTANCE, 1999, 5 (01) :53-56
[6]   Avoparcin used as a growth promoter is associated with the occurrence of vancomycin-resistant Enterococcus faecium on Danish poultry and pig farms [J].
Bager, F ;
Madsen, M ;
Christensen, J ;
Aarestrup, FM .
PREVENTIVE VETERINARY MEDICINE, 1997, 31 (1-2) :95-112
[7]   DANMAP: monitoring antimicrobial resistance in Denmark [J].
Bager, F .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2000, 14 (04) :271-274
[8]   EVIDENCE FOR AN ANIMAL ORIGIN OF VANCOMYCIN-RESISTANT ENTEROCOCCI [J].
BATES, J ;
JORDENS, Z ;
SELKON, JB .
LANCET, 1993, 342 (8869) :490-491
[9]  
Borgen K, 2000, FEMS MICROBIOL LETT, V191, P255, DOI 10.1111/j.1574-6968.2000.tb09348.x
[10]   Continuing high prevalence of VanA-type vancomycin-resistant enterococci on Norwegian poultry farms three years after avoparcin was banned [J].
Borgen, K ;
Simonsen, GS ;
Sundsfjord, A ;
Wasteson, Y ;
Olsvik, O ;
Kruse, H .
JOURNAL OF APPLIED MICROBIOLOGY, 2000, 89 (03) :478-485