Fluorescent ion-selective nanosensors for intracellular analysis with improved lifetime and size

被引：61

作者：

Dubach, J. Matthew ^{[1
]}

Harjes, Daniel I. ^{[1
]}

Clark, Heather A. ^{[1
]}

机构：

[1] Charles Stark Draper Lab Inc, Bioengn Grp, Cambridge, MA 02139 USA

来源：

NANO LETTERS | 2007年 / 7卷 / 06期

关键词：

D O I：

10.1021/nl0707860

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the synthesis and characterization of sodium-selective polymeric nanosensors that improves upon the lifetime and size of previous fiberless nanosensors. Sonication is used to form the polymer nanospheres that contain all the components needed for ion sensing. Even though the size is small (approximately 120 nm), the lifetime of these sensors in solution is on the order of a week. The surface coating has also been optimized for stability, biocompatibility, and ease of chemical modification.

引用

页码：1827 / 1831

页数：5

共 18 条

[1]

[Anonymous], P IEEE

[2] Carrier-based ion-selective electrodes and bulk optodes. 1. General characteristics [J].

Bakker, E ;

Buhlmann, P ;

Pretsch, E .

CHEMICAL REVIEWS, 1997, 97 (08) :3083-3132

[3] Astrocytes generate Na+-mediated metabolic waves [J].

Bernardinelli, Y ;

Magistretti, PJ ;

Chatton, JY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (41) :14937-14942

[4] Fluorescent nanosensors for intracellular chemical analysis: Decyl methacrylate liquid polymer matrix and ion exchange-based potassium PEBBLE sensors with real-time application to viable rat C6 glioma cells [J].

Brasuel, M ;

Kopelman, R ;

Miller, TJ ;

Tjalkens, R ;

Philbert, MA .

ANALYTICAL CHEMISTRY, 2001, 73 (10) :2221-2228

[5] Liquid polymer nano-PEBBLEs for Cl- analysis and biological applications [J].

Brasuel, MG ;

Miller, TJ ;

Kopelman, R ;

Philbert, MA .

ANALYST, 2003, 128 (10) :1262-1267

[6] Carrier-based ion-selective electrodes and bulk optodes. 2. Ionophores for potentiometric and optical sensors [J].

Buhlmann, P ;

Pretsch, E ;

Bakker, E .

CHEMICAL REVIEWS, 1998, 98 (04) :1593-1687

[7] Optical nanosensors for chemical analysis inside single living cells. 1. Fabrication, characterization, and methods for intracellular delivery of PEBBLE sensors [J].

Clark, HA ;

Hoyer, M ;

Philbert, MA ;

Kopelman, R .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4831-4836

[8] Optical nanosensors for chemical analysis inside single living cells. 2. Sensors for pH and calcium and the intracellular application of PEBBLE sensors [J].

Clark, HA ;

Kopelman, R ;

Tjalkens, R ;

Philbert, MA .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4837-4843

[9] High-content kinetic calcium imaging in drug-sensitive and drug-resistant human breast cancer cells [J].

DeBernardi, Maria A. ;

Brooker, Gary .

MEASURING BIOLOGICAL RESPONSES WITH AUTOMATED MICROSCOPY, 2006, 414 :317-335

[10] A novel method for analyzing [Ca2+] flux kinetics in high-throughput screening [J].

Gribbon, Philip ;

Chambers, Chris ;

Palo, Kaupo ;

Kupper, Juergen ;

Mueller, Juergen ;

Sewing, Andreas .

JOURNAL OF BIOMOLECULAR SCREENING, 2006, 11 (05) :511-518

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