Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers

被引：96

作者：

Amicucci, A

Zambonelli, A

Giomaro, G

Potenza, L

Stocchi, V

机构：

[1] Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, Italy

[2] Univ Bologna, Dipartimento Prot & Valorizzaz Agroalimentare, I-40126 Bologna, Italy

[3] Univ Urbino, Ist Orto Bot, I-61029 Urbino, Italy

来源：

MOLECULAR ECOLOGY | 1998年 / 7卷 / 03期

关键词：

ectomycorrhiza; identification; ITS; SCAR marker; specific PCR; Tuber;

D O I：

10.1046/j.1365-294X.1998.00357.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study reports PCR-based techniques for a reliable molecular identification of five species of white truffles: Tuber magnatum Pico, T. borchii Vittad., T. maculatum Vittad., T. dryophilum Berk. & Br. and T. puberulum Perk. & Br. The sequences of the ITS region of several Tuber spp. were analysed and a pair of primers was designed for each species under study. The selected pairs of specific primers can be used for simple, rapid, reliable and unambiguous identification during the three developmental phases of the truffle life cycle: fruitbody, mycelium and ectomycorrhiza.

引用

页码：273 / 277

页数：5

共 13 条

[1] Use of sequence characterised amplified region and RAPD markers in the identification of the white truffle Tuber magnatum Pico [J].