Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy

被引：160

作者：

Gautier, Antoine ^{[1
]}

Mott, Helen R. ^{[1
]}

Bostock, Mark J. ^{[1
]}

Kirkpatrick, John P. ^{[1
]}

Nietlispach, Daniel ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2010年 / 17卷 / 06期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

PROTEIN-COUPLED RECEPTORS; RESIDUAL DIPOLAR COUPLINGS; NUCLEAR-MAGNETIC-RESONANCE; INTEGRAL MEMBRANE ENZYME; LARGE-SCALE PRODUCTION; X-RAY-STRUCTURE; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; FUNCTIONAL EXPRESSION; CHEMICAL-SHIFT;

D O I：

10.1038/nsmb.1807

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 angstrom) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors.

引用

收藏

页码：768 / U147

页数：8

相关论文

共 58 条

[31] Crystal structure of sensory rhodopsin II at 2.4 angstroms: Insights into color tuning and transducer interaction [J].

Luecke, H ;

Schobert, B ;

Lanyi, JK ;

Spudich, EN ;

Spudich, JL .

SCIENCE, 2001, 293 (5534) :1499-1503

[32] Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor [J].

Magnani, Francesca ;

Shibata, Yoko ;

Serrano-Vega, Maria J. ;

Tate, Christopher G. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (31) :10744-10749

[33] Heterologous GPCR expression: A bottleneck to obtaining crystal structures [J].

McCusker, Emily C. ;

Bane, Steven E. ;

O'Malley, Michelle A. ;

Robinson, Anne Skaja .

BIOTECHNOLOGY PROGRESS, 2007, 23 (03) :540-547

[34] Structure and Dynamics of Membrane Proteins by Magic Angle Spinning Solid-State NMR [J].

McDermott, Ann .

ANNUAL REVIEW OF BIOPHYSICS, 2009, 38 :385-403

[35] Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies [J].

Michalke, Kerstin ;

Graviere, Marie-Eve ;

Huyghe, Celine ;

Vincentelli, Renaud ;

Wagner, Renaud ;

Pattus, Franc ;

Schroeder, Kathrin ;

Oschmann, Jan ;

Rudolph, Rainer ;

Cambillau, Christian ;

Desmyter, Aline .

ANALYTICAL BIOCHEMISTRY, 2009, 386 (02) :147-155

[36] The structure of phospholamban pentamer reveals a channel-like architecture in membranes [J].

Oxenoid, K ;

Chou, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (31) :10870-10875

[37] Isotropic bicelles stabilize the functional form of a small multidrug-resistance pump for NMR structural studies [J].

Poget, Sebastien F. ;

Cahill, Sean M. ;

Girvin, Mark E. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (09) :2432-+

[38] Crystal structure of the human β2 adrenergic G-protein-coupled receptor [J].

Rasmussen, Soren G. F. ;

Choi, Hee-Jung ;

Rosenbaum, Daniel M. ;

Kobilka, Tong Sun ;

Thian, Foon Sun ;

Edwards, Patricia C. ;

Burghammer, Manfred ;

Ratnala, Venkata R. P. ;

Sanishvili, Ruslan ;

Fischetti, Robert F. ;

Schertler, Gebhard F. X. ;

Weis, William I. ;

Kobilka, Brian K. .

NATURE, 2007, 450 (7168) :383-U4

[39] Conformational changes associated with receptor-stimulated guanine nucleotide exchange in a heterotrimeric G-protein α-subunit -: NMR analysis of GTPγS-bound states [J].

Ridge, KD ;

Abdulaev, NG ;

Zhang, C ;

Ngo, T ;

Brabazon, DM ;

Marino, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (11) :7635-7648

[40] Accelerated acquisition of high resolution triple-resonance spectra using non-uniform sampling and maximum entropy reconstruction [J].

Rovnyak, D ;

Frueh, DP ;

Sastry, M ;

Sun, ZYJ ;

Stern, AS ;

Hoch, JC ;

Wagner, G .

JOURNAL OF MAGNETIC RESONANCE, 2004, 170 (01) :15-21

← 1 2 3 4 5 6 →