Experiences and perspectives of MALDI MS and MS/MS in proteomic research

被引:43
作者
Rappsilber, J
Moniatte, M
Nielsen, ML
Podtelejnikov, AV
Mann, M
机构
[1] Univ So Denmark, Ctr Expt Bioinformat, Prot Interact Lab, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark
[2] MDS Prote AS, DK-5230 Odense M, Denmark
关键词
MALDI; electrospray; proteomics;
D O I
10.1016/S1387-3806(02)00976-4
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
The simplicity, robustness, and sensitivity of MALDI have made it a fundamental technique of proteomics. Here we report on our experience in high throughput protein identification by MALDI peptide mass fingerprinting. A streamlined system has been developed that encompasses spot excision, digestion and sample/matrix preparation, data acquisition, database searching and logging of the results. Despite achieving robust and high throughput identification we find that MALDI peptide mass fingerprinting alone is not sufficiently selective for current demands in proteomic research. However, the combination of MALDI with tandem mass spectrometry such as joining a quadrupole with a TOF analyser pairs highly parallel analysis with high selectivity. Thus MALDI will remain an important technique in the modem proteomic laboratory. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:223 / 237
页数:15
相关论文
共 72 条
[1]   ALPHA-CYANO-4-HYDROXYCINNAMIC ACID AS A MATRIX FOR MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY [J].
BEAVIS, RC ;
CHAUDHARY, T ;
CHAIT, BT .
ORGANIC MASS SPECTROMETRY, 1992, 27 (02) :156-158
[2]   Proteomics characterization of abundant Golgi membrane proteins [J].
Bell, AW ;
Ward, MA ;
Blackstock, WP ;
Freeman, HNM ;
Choudhary, JS ;
Lewis, AP ;
Chotai, D ;
Fazel, A ;
Gushue, JN ;
Paiement, J ;
Palcy, S ;
Chevet, E ;
Lafrenière-Roula, M ;
Solari, R ;
Thomas, DY ;
Rowley, A ;
Bergeron, JJM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (07) :5152-5165
[3]  
Brancia FL, 2000, RAPID COMMUN MASS SP, V14, P2070, DOI 10.1002/1097-0231(20001115)14:21<2070::AID-RCM133>3.0.CO
[4]  
2-G
[5]   SEQUENCE-SPECIFIC FRAGMENTATION OF MATRIX-ASSISTED LASER-DESORBED PROTEIN PEPTIDE IONS [J].
BROWN, RS ;
LENNON, JJ .
ANALYTICAL CHEMISTRY, 1995, 67 (21) :3990-3999
[6]   Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry [J].
Chaurand, P ;
Luetzenkirchen, F ;
Spengler, B .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1999, 10 (02) :91-103
[7]   Duty cycle improvement for a quadrupole-time-of-flight mass spectrometer and its use for precursor ion scans [J].
Chernushevich, IV .
EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2000, 6 (06) :471-479
[8]   IMPROVING THE RESOLUTION OF MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY BY EXPLOITING THE CORRELATION BETWEEN ION POSITION AND VELOCITY [J].
COLBY, SM ;
KING, TB ;
REILLY, JP .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (11) :865-868
[9]   Improvements in protein identification by MALDI-TOF-MS peptide mapping [J].
Egelhofer, V ;
Büssow, K ;
Luebbert, C ;
Lehrach, H ;
Nordhoff, E .
ANALYTICAL CHEMISTRY, 2000, 72 (13) :2741-2750
[10]   AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].
ENG, JK ;
MCCORMACK, AL ;
YATES, JR .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989