Differentiation of Healthy Brain Tissue and Tumors Using Surface-Enhanced Raman Scattering

被引：59

作者：

Aydin, Oemer ^{[1
]}

Altas, Murat ^{[2
]}

Kahraman, Mehmet ^{[1
]}

Bayrak, Oemer Faruk ^{[1
]}

Culha, Mustafa ^{[1
]}

机构：

[1] Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, TR-34755 Kayisdagi Istanbul, Turkey

[2] Dr Lutfi Kirdar Kartal Educ & Res Hosp, Istanbul, Turkey

来源：

APPLIED SPECTROSCOPY | 2009年 / 63卷 / 10期

关键词：

Surface-enhanced Raman scattering; SERS; Tissue differentiation; Brain tumor; Diagnosis; Silver nanoparticles; SPECTROSCOPY; DISCRIMINATION; CLASSIFICATION; SERS; IDENTIFICATION; NANOPARTICLES; PROTEINS; PYRIDINE; SPECTRA; TOOL;

D O I：

10.1366/000370209789553219

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Surface-enhanced Raman scattering (SERS) is a powerful technique for characterization of biological samples. SERS spectra from healthy brain tissue and tumors are obtained by sudden freezing of tissue in liquid nitrogen and crashing and mixing it with a concentrated silver colloidal suspension. The acquired spectra from tissues show significant spectral differences that can be used to identify whether it is from a healthy region or tumor. The most significant change on SERS spectra from the healthy/peripheral brain tissue to tumor is the increase of the ratio of the peaks at around 723 to 655 cm(-1). In addition, the spectral changes indicate that the protein content in tumors increases compared to the peripheral/healthy tissue as observed with tumor invasion. The preliminary results show that SERS spectra can be used for a quick diagnosis due to the simplicity of the sample preparation and the speed of the spectral acquisition.

引用

页码：1095 / 1100

页数：6

共 47 条

[1] ANOMALOUSLY INTENSE RAMAN-SPECTRA OF PYRIDINE AT A SILVER ELECTRODE [J].

ALBRECHT, MG ;

CREIGHTON, JA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (15) :5215-5217

[2] Discriminating healthy from tumor and necrosis tissue in rat brain tissue samples by Raman spectral imaging [J].

Amharref, Nadia ;

Bejebbar, Abdelilah ;

Dukie, Sylvain ;

Venteo, Lydie ;

Schneider, Laurence ;

Pluot, Michel ;

Manfait, Michel .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (10) :2605-2615

[3] Locally enhanced Raman spectroscopy with an atomic force microscope [J].

Anderson, MS .

APPLIED PHYSICS LETTERS, 2000, 76 (21) :3130-3132

[4] Study of normal colorectal tissue by FT-Raman spectroscopy [J].

Andrade, P. O. ;

Bitar, R. A. ;

Yassoyama, K. ;

Martinho, H. ;

Santo, A. M. E. ;

Bruno, P. M. ;

Martin, A. A. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (05) :1643-1648

[5] Surface-Enhanced Raman Scattering of Rat Tissues [J].

Aydin, Oemer ;

Kahraman, Mehmet ;

Kilic, Ertugrul ;

Culha, Mustafa .

APPLIED SPECTROSCOPY, 2009, 63 (06) :662-668

[6]

Caspers PJ, 1998, BIOSPECTROSCOPY, V4, pS31, DOI 10.1002/(SICI)1520-6343(1998)4:5+<S31::AID-BSPY4>3.0.CO

[7]

2-M

[8] Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells [J].

Chan, JW ;

Taylor, DS ;

Zwerdling, T ;

Lane, SM ;

Ihara, K ;

Huser, T .

BIOPHYSICAL JOURNAL, 2006, 90 (02) :648-656

[9] Surface-enhanced Raman spectroscopy using self-assembled silver nanoparticles on silicon nanotips [J].

Chattopadhyay, S ;

Lo, HC ;

Hsu, CH ;

Chen, LC ;

Chen, KH .

CHEMISTRY OF MATERIALS, 2005, 17 (03) :553-559

[10] Use of surface-enhanced Raman spectroscopy for the detection of human integrins [J].

Chowdhury, Mustafa H. ;

Gant, V. Alexander ;

Trache, Andreea ;

Baldwin, Angela ;

Meininger, Gerald. A. ;

Cote, Gerard L. .

JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (02)

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