Functional and molecular expression of PACAP/VIP receptors in the rat retina

被引：49

作者：

D'Agata, V ^{[1
]}

Cavallaro, S ^{[1
]}

机构：

[1] CNR, Ist Bioimmagini & Fisiopatol Sistema Nervoso Cent, I-95123 Catania, Italy

来源：

MOLECULAR BRAIN RESEARCH | 1998年 / 54卷 / 01期

关键词：

pituitary adenylate cyclase activating polypeptide (PACAP); vasoactive intestinal peptide (VIP); receptor; retina; mRNA; inositol phospholipid hydrolysis; cAMP;

D O I：

10.1016/S0169-328X(97)00335-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Receptor binding sites for pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP), positively coupled to adenylate cyclase, have been previously described in the retina of different mammalian species. In the present study, we determined the mRNA expression of PACAP/VIP receptor variants in the rat retina and investigated their coupling to phospholipase C in addition to adenylate cyclase. The two forms of PACAP, PACAP27 and PACAP38, induced a dose-dependent (1-100 nM) increase of cAMP and [H-3]inositol monophosphate levels, whereas VIP stimulated, with lower potency and efficacy, cAMP formation only. Reverse transcription-PCR analysis in the rat retina detected both type-I (PACAP-R and PACAP-HOP splice variants) and type-II(VIP-l and -2) receptor-mRNAs. These data indicate that PACAP and VIP may interact with multiple receptor subtypes and activate one (VIP) or two (PACAP) signal transduction mechanisms in the rat retina. (C) 1998 Elsevier Science B.V.

引用

页码：161 / 164

页数：4

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