Numerical investigation of an electrochemically induced tagging in a nanospray for protein analysis

被引：22

作者：

Rohner, TC ^{[1
]}

Josserand, J ^{[1
]}

Jensen, H ^{[1
]}

Girault, HH ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chim Mol & Biol, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 09期

关键词：

D O I：

10.1021/ac026270c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An on-line tagging of target species is simulated using the finite element method. A numerical model of an electrochemical EC2XE mechanism in a flow channel cell has been developed, corresponding to the electrochemical generation of a probe and the subsequent homogeneous reaction with the target. The kinetic and convective aspects are validated on short electrode geometries before taking into account the depletion of the target species. The model is then assessed according to previous experimental results on the on-line tagging of proteins. The occurrence and the efficiency of the on-line tagging are studied for both pressure-driven and electroosmotic flows. The involved phenomena including kinetic aspects are described in detail. Finally, optimal conditions for an effective quantitative tagging are discussed.

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页码：2065 / 2074

页数：10

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