Characterization and chromosomal mapping of antimicrobial resistance genes in Salmonella enterica serotype Typhimurium

被引：52

作者：

Daly, M ^{[1
]}

Fanning, S ^{[1
]}

机构：

[1] Cork Inst technol, Mol Diagnost Unit, Cork, Ireland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 11期

关键词：

D O I：

10.1128/AEM.66.11.4842-4848.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two hundred and twenty-six Salmonella enterica serotype Typhimurium isolates were examined for the presence of integron-associated gene cassettes. All but two of the non-DT104? isolates, together with DTL04 isolates, contained gene cassettes, Amplicons of 1.5 kbp each were found in tyro non-DT104 isolates, encoding a dhfrI gene (trimethoprim resistance) linked to an aad4 gene (streptomycin and spectinomycin resistance), by site-specific recombination, DT104 isolates of resistance (R) type ACSSuT possessed the recently described 1.0-and 1.2-kbp gene cassettes. Macrorestriction analysis with XbaI and DNA probing mapped ant(3")-1a,bla(PSE-1) and dhfrI genes to large multiresistant gene clusters in a DT170a isolate and a DT193 isolate. In contrast, all DT104 isolates (R-type ACSSuT) possessed a conserved 10-kbp Xba1 DNA fragment, Our study highlights the occurrence of integrons (and antimicrobial resistance determinants) among serotype Typhimurium isolates other than DT104. Larger and previously unrecognized multiresistance gene clusters were identified in these isolates by DNA probing.

引用

页码：4842 / +

页数：8

共 32 条

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