Single-molecule folding

被引：167

作者：

Zhuang, XW ^{[1
]}

Rief, M

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

[2] Lehrstuhl Angew Phys, D-80799 Munich, Germany

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2003年 / 13卷 / 01期

关键词：

D O I：

10.1016/S0959-440X(03)00011-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in fluorescence and force spectroscopy enable us to go beyond the ensemble average and measure the behavior of individual biomacromolecules. These single-molecule approaches can directly resolve transient intermediate states and multiple reaction pathways, and thus are uniquely powerful in characterizing the complex dynamics of biological processes. Recent applications of these two techniques to the protein and RNA folding problems have led to exciting new results.

引用

页码：88 / 97

页数：10

共 57 条

[1] Pathways and intermediates in forced unfolding of spectrin repeats
Altmann, SM
Grünberg, RG
Lenne, PF
Ylänne, J
Raae, A
Herbert, K
Saraste, M
Nilges, M
Hörber, JKH
[J]. STRUCTURE, 2002, 10 (08) : 1085 - 1096
[2] PROTEIN-FOLDING - MATCHING SPEED AND STABILITY
BALDWIN, RL
[J]. NATURE, 1994, 369 (6477) : 183 - 184
[3] BELL GI, 1978, SCIENCE, V200, P618, DOI 10.1126/science.347575
[4] A simple method for probing the mechanical unfolding pathway of proteins in detail
Best, RB
Fowler, SB
Toca-Herrera, JL
Clarke, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (19) : 12143 - 12148
[5] Can non-mechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation
Best, RB
Li, B
Steward, A
Daggett, V
Clarke, J
[J]. BIOPHYSICAL JOURNAL, 2001, 81 (04) : 2344 - 2356
[6] Single-molecule fluorescence resonant energy transfer in calcium concentration dependent cameleon
Brasselet, S
Peterman, EJG
Miyawaki, A
Moerner, WE
[J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (15): : 3676 - 3682
[7] The effect of core destabilization on the mechanical resistance of I27
Brockwell, DJ
Beddard, GS
Clarkson, J
Zinober, RC
Blake, AW
Trinick, J
Olmsted, PD
Smith, DA
Radford, SE
[J]. BIOPHYSICAL JOURNAL, 2002, 83 (01) : 458 - 472
[8] Mechanical and chemical unfolding of a single protein: A comparison
Carrion-Vazquez, M
Oberhauser, AF
Fowler, SB
Marszalek, PE
Broedel, SE
Clarke, J
Fernandez, JM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) : 3694 - 3699
[9] Force spectroscopy with single bio-molecules
Clausen-Schaumann, H
Seitz, M
Krautbauer, R
Gaub, HE
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (05) : 524 - 530
[10] Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2
Deniz, AA
Laurence, TA
Beligere, GS
Dahan, M
Martin, AB
Chemla, DS
Dawson, PE
Schultz, PG
Weiss, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) : 5179 - 5184

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