In vivo single-shot three-dimensionally localized multiple quantum spectroscopy of GABA in the human brain with improved spectral selectivity

A single-shot multiple quantum filtering method is developed that uses two doubie-band frequency selective pulses enhanced spectral selectivity in combination with a slice-selective 90degrees, a slice-selective universal rotator 90degrees, and a spectral-spatial pulse composed of two slice-selective universal rotator 45degrees pulses for single-shot three-dimensional localization. The use of ibis selective multiple quantum filtering method for C-3 and C-4 methylene protons of GABA resulted in improved spectral selectivity for GABA and effective suppression of overlapping signals Such as creatine and glutathione in each single scan, providing reliable measurement of the GABA doublet in all subjects. The concentration of GABA was measured to be 0.7 +/- 0.2 mumol/g (means +/- SD, n = 15) in the fronto-parietal region of the human brain in vivo. (C) 2004 Elsevier Inc. All rights reserved.