Update on Pneumocystis carinii f. sp. hominis typing based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes

被引:118
作者
Lee, CH
Helweg-Larsen, J
Tang, X
Jin, SL
Li, BZ
Bartlett, MS
Lu, JJ
Lundgren, B
Lundgren, JD
Olsson, M
Lucas, SB
Roux, P
Cargnel, A
Atzori, C
Matos, O
Smith, JW
机构
[1] Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA
[2] Univ Copenhagen, Hvidovre Hosp, Dept Infect Dis, Copenhagen, Denmark
[3] Univ Copenhagen, Hvidovre Hosp, Dept Clin Microbiol, Copenhagen, Denmark
[4] Natl Def Med Ctr, Taipei, Taiwan
[5] Tri Serv Gen Hosp, Dept Pathol, Taipei, Taiwan
[6] Swedish Inst Infect Dis Control, Stockholm, Sweden
[7] St Thomas Hosp, Dept Histopathol, London SE1 7EH, England
[8] Univ St Antoine, Ctr Hosp, Lab Parasitol Mycol, Paris, France
[9] Osped L Sacco, Div Malattie Infett 2, Milan, Italy
[10] Inst Higiene & Med Trop, Lisbon, Portugal
关键词
D O I
10.1128/JCM.36.3.734-741.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pneumocystis carinii f. sp. hominis isolates from 207 clinical specimens from nine countries were typed based on nucleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, respectively) of rRNA genes. The number of ITS1 nucleotides has been revised from the previously reported 157 bp to 161 bp. Likewise, the number of ITS2 nucleotides has been changed from 177 to 192 bp. The number of ITS1 sequence types has increased from 2 to 15, and that of ITS2 has increased from 3 to 14. The 15 ITS1 sequence types are designated types A through O, and the 14 ITS2 types are named types a through n. A total of 59 types of P. carinii f. sp. hominis were found in this study.
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页码:734 / 741
页数:8
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