NLS bioconjugates for targeting therapeutic genes to the nucleus

被引：134

作者：

Escriou, V

Carrière, M

Scherman, D

Wils, P

机构：

[1] Gencell SA, F-94400 Vitry Sur Seine, France

[2] Ctr Rech Vitry Alfortville, Aventis, ENSCP, CNRS,UMR 7001, F-94403 Vitry Sur Seine, France

来源：

ADVANCED DRUG DELIVERY REVIEWS | 2003年 / 55卷 / 02期

关键词：

gene transfer; nuclear import; plasmid DNA; non-viral;

D O I：

10.1016/S0169-409X(02)00184-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

One of the major steps limiting non-viral gene transfer efficiency is the entry of plasmid DNA from the cytoplasm into the nucleus of transfected cells. Trafficking of nuclear proteins from the cytoplasm into the nucleus through nuclear pore complexes is mediated by the presence of nuclear localization sequences (NLS) on proteins. Viral DNA and RNA also require interaction with cellular machinery for efficient nuclear import. In this article, we review the various strategies used to provide plasmid DNA with nuclear localization sequences, and discuss the possibility of developing efficient gene delivery systems based on these strategies. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：295 / 306

页数：12

共 45 条

[1] Nuclear localization signal peptides enhance cationic liposome-mediated gene therapy [J].