Determination of catechins in human plasma after commercial canned green tea ingestion by high-performance liquid chromatography with electrochemical detection using a microbore column

被引：53

作者：

Kotani, A ^{[1
]}

Miyashita, N ^{[1
]}

Kusu, F ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Hachioji, Tokyo 1920392, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 788卷 / 02期

关键词：

catechins; (-)-EPIGALLOCATECHIN GALLATE; LIPID-PEROXIDATION; POLYPHENOLS; TANNINS; HPLC;

D O I：

10.1016/S1570-0232(02)01036-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determination of catechins in 'human plasma was carried out by high-performance liquid chromatography with electrochemical detection using a microbore octadecylsilica column. Peak heights for catechins were found to be linearly related to the amount of each catechin injected, from 2 pmol/ml to 2 nmol/ml (r>0.999). Conjugated-form catechins in plasma were hydrolyzed enzymatically using beta-glucuronidase and sulfatase. Catechins in plasma and the hydrolyzed solution were extracted with ethyl acetate and determined by the present method. The time courses of concentrations of catechins in human plasma showed maxima at 1-2 h after ingestion of 340 ml of commercial canned green tea. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：269 / 275

页数：7

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