Efficient Generation of Germ Line Transmitting Chimeras from C57BL/6N ES Cells by Aggregation with Outbred Host Embryos

被引：83

作者：

Gertsenstein, Marina ^{[1
,2
]}

Nutter, Lauryl M. J. ^{[3
]}

Reid, Tammy ^{[4
]}

Pereira, Monica ^{[1
,2
]}

Stanford, William L. ^{[4
]}

Rossant, Janet ^{[3
,5
,6
]}

Nagy, Andras ^{[1
,5
]}

机构：

[1] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[2] Toronto Ctr Phenogen, Toronto, ON, Canada

[3] Hosp Sick Children, Toronto, ON M5G 1X8, Canada

[4] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON, Canada

[5] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[6] Univ Toronto, Dept Obstet & Gynaecol, Toronto, ON, Canada

来源：

PLOS ONE | 2010年 / 5卷 / 06期

基金：

美国国家卫生研究院;

关键词：

INBRED MOUSE STRAINS; STEM-CELLS; BALB/C BLASTOCYSTS; RAT BLASTOCYSTS; SELF-RENEWAL; MICE; GENE; ESTABLISHMENT; KNOCKOUT; DERIVATION;

D O I：

10.1371/journal.pone.0011260

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every gene of the mouse genome. To realize the utility of these resources, efficient and accessible methods of generating mutant mice from these ES cells are necessary. Here, we describe a combination of ICR morula aggregation and a chemically-defined culture medium with widely available and accessible components for the high efficiency generation of germline transmitting chimeras from C57BL/6N ES cells. Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources.

引用

页数：8

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