Recombinant protein solubility - does more mean better?

被引：103

作者：

Gonzalez-Montalban, Nuria

Garcia-Fruitos, Elena

Villaverde, Antonio ^{[1
]}

机构：

[1] Autonomous Univ Barcelona, Dept Genet & Microbiol, Inst Biotechnol & Biomed, Bellaterra 08193, Barcelona, Spain

[2] Autonomous Univ Barcelona, CIBER Bioengn Biomat & Nanomed, Bellaterra 08193, Barcelona, Spain

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 07期

关键词：

D O I：

10.1038/nbt0707-718

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页码：718 / 720

页数：4

共 16 条

[1] Formation of active inclusion bodies in the periplasm of Escherichia coli [J].

Arie, Jean-Philippe ;

Miot, Marika ;

Sassoon, Nathalie ;

Betton, Jean-Michel .

MOLECULAR MICROBIOLOGY, 2006, 62 (02) :427-437

[2] Recombinant protein folding and misfolding in Escherichia coli [J].

Baneyx, F ;

Mujacic, M .

NATURE BIOTECHNOLOGY, 2004, 22 (11) :1399-1408

[3] Amyloid-like properties of bacterial inclusion bodies [J].

Carrió, M ;

González-Montalbán, N ;

Vera, A ;

Villaverde, A ;

Ventura, S .

JOURNAL OF MOLECULAR BIOLOGY, 2005, 347 (05) :1025-1037

[4] Localization of chaperones DnaK and GroEL in bacterial inclusion bodies [J].

Carrió, MM ;

Villaverde, A .

JOURNAL OF BACTERIOLOGY, 2005, 187 (10) :3599-3601

[5] Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates [J].

de Groot, Natalia Sanchez ;

Ventura, Salvador .

JOURNAL OF BIOTECHNOLOGY, 2006, 125 (01) :110-113

[6]

DEMARCO A, 2005, BMC BIOCH, V6, P10

[7] Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins -: art. no. 27 [J].

García-Fruitós, E ;

González-Montalbán, N ;

Morell, M ;

Vera, A ;

Ferraz, RM ;

Arís, A ;

Ventura, S ;

Villaverde, A .

MICROBIAL CELL FACTORIES, 2005, 4 (1)

[8] Folding of a misfolding-prone β-galactosidase in absence of DnaK [J].

García-Fruitós, E ;

Carrió, MM ;

Arís, A ;

Villaverde, A .

BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (07) :869-875

[9] Localization of functional polypeptides in bacterial inclusion bodies [J].

Garcia-Fruitos, Elena ;

Aris, Anna ;

Villaverde, Antonio .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (01) :289-294

[10] The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells [J].

Gonzalez-Montalban, Nuria ;

Garcia-Fruitos, Elena ;

Ventura, Salvador ;

Aris, Anna ;

Villaverde, Antonio .

MICROBIAL CELL FACTORIES, 2006, 5 (1)

← 1 2 →