Molecular identification of Paracoccidioides brasiliensis by 5′ nuclease assay

被引：21

作者：

Semighini, CP

de Camargo, ZP

Puccia, R

Goldman, MHS

Goldman, GH ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP, Brazil

[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil

[3] USP, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-09500900 Sao Paulo, SP, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2002年 / 44卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/S0732-8893(02)00472-8

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：383 / 386

页数：4

共 17 条

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