Hyaluronan mediates adhesion of metastatic colon carcinoma cells

被引:35
作者
Laurich, C
Wheeler, MA
Iida, J
Neudauer, CL
McCarthy, JB
Bullard, KM
机构
[1] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
关键词
hyaluronan; hyaluronan synthase; colon carcinoma; colorectal cancer; adhesion; laminin; extracellular matrix;
D O I
10.1016/j.jss.2004.05.018
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Hyaluronan (HA) is a cell-surface glycosaminoglycan that has been implicated in cancer progression. Cells isolated from metastatic colon carcinoma (SW620) produce greater amounts of pericellular HA than cells isolated from a primary tumor (SW480). Inhibition of hyaluronan synthases (HAS) by transfection with antisense cDNA decreases HA production. Because adhesion to the extracellular matrix (ECM) is required for invasion and metastasis, we hypothesized that pericellular HA mediates adhesion to ECM proteins such as laminin, collagen, and fibronectin and that inhibition of HA production or removal of HA by digestion with hyaluronidase would impair adhesion. Materials and methods. SW480, SW620, and antisense transfectants (SW620 cells transfected with vector alone, antisense HAS2, antisense HAS3, and both antisense HAS2 and HAS3) were assessed for adhesion to laminin, Type 1 collagen, or fibronectin-coated plates. To confirm that adhesion was mediated by HA, cells were treated with or without hyaluronidase prior to the assays. Results. Metastatic SW620 cells adhered well to laminin; SW480 cells demonstrated 46% less adhesion (P < 0.05; Student's t test). SW620 cell adhesion to Type 1 collagen and fibronectin was >50% less than adhesion to laminin. Inhibition of HAS2 and/or HAS3 or pretreatment with hyaluronidase significantly decreased adhesion of SW620 cells to laminin (P < 0.05), suggesting that adhesion was dependent upon pericellular HA. Conclusions. Metastatic SW620 cells that produce large amounts of pericellular HA adhered well to laminin. Inhibition of HAS2 and/or HAS3 expression, or hyaluronidase digestion of pericellular HA significantly inhibited adhesion. These data suggest that HA promotes adhesion to laminin and may thereby facilitate invasion of the basement membrane and metastasis in colon carcinoma. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:70 / 74
页数:5
相关论文
共 42 条
[1]   CD44 is the principal mediator of hyaluronic-acid-induced melanoma cell proliferation [J].
Ahrens, T ;
Assmann, V ;
Fieber, C ;
Termeer, CC ;
Herrlich, P ;
Hofmann, M ;
Simon, JC .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 116 (01) :93-101
[2]   Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is involved in the activation of endothelial Integrins [J].
Akada, T ;
Yamazaki, T ;
Miyashita, H ;
Niizeki, O ;
Abe, M ;
Sato, A ;
Satomi, S ;
Sato, Y .
JOURNAL OF CELLULAR PHYSIOLOGY, 2002, 193 (02) :253-262
[3]  
Anttila MA, 2000, CANCER RES, V60, P150
[4]   The pattern of expression of the microtubule-binding protein RHAMM/IHABP in mammary carcinoma suggests a role in the invasive behaviour of tumour cells [J].
Assmann, V ;
Gillett, CE ;
Poulsom, R ;
Ryder, K ;
Hart, IR ;
Hanby, AM .
JOURNAL OF PATHOLOGY, 2001, 195 (02) :191-196
[5]   Hyaluronan in peritumoral stroma and malignant cells associates with breast cancer spreading and predicts survival [J].
Auvinen, P ;
Tammi, R ;
Parkkinen, J ;
Tammi, M ;
Ågren, U ;
Johansson, R ;
Hirvikoski, P ;
Eskelinen, M ;
Kosma, VM .
AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (02) :529-536
[6]   LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan [J].
Banerji, S ;
Ni, J ;
Wang, SX ;
Clasper, S ;
Su, J ;
Tammi, R ;
Jones, M ;
Jackson, DG .
JOURNAL OF CELL BIOLOGY, 1999, 144 (04) :789-801
[7]   Up-regulation and differential expression of the hyaluronan-binding protein TSG-6 in cartilage and synovium in rheumatoid arthritis and osteoarthritis [J].
Bayliss, MT ;
Howat, SLT ;
Dudhia, J ;
Murphy, JM ;
Barry, FP ;
Edwards, JCW ;
Day, AJ .
OSTEOARTHRITIS AND CARTILAGE, 2001, 9 (01) :42-48
[8]   Layilin, a novel integral membrane protein, is a hyaluronan receptor [J].
Bono, P ;
Rubin, K ;
Higgins, JMG ;
Hynes, RO .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (04) :891-900
[9]   Hyaluronan synthase-3 is upregulated in metastatic colon carcinoma cells and manipulation of expression alters matrix retention and cellular growth [J].
Bullard, KM ;
Kim, HR ;
Wheeler, MA ;
Wilson, CM ;
Neudauer, CL ;
Simpson, MA ;
McCarthy, JB .
INTERNATIONAL JOURNAL OF CANCER, 2003, 107 (05) :739-746
[10]   EMMPRIN (extracellular matrix metalloproteinase inducer) is a novel marker of poor outcome in serous ovarian carcinoma [J].
Davidson, B ;
Goldberg, I ;
Berner, A ;
Kristensen, GB ;
Reich, R .
CLINICAL & EXPERIMENTAL METASTASIS, 2003, 20 (02) :161-169