Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins

被引：20

作者：

Amoroso, MG

Longobardo, L

Capparelli, R

机构：

[1] Univ Naples Federico II, Sch Biotechnol Sci, I-80055 Naples, Italy

[2] Univ Naples Federico II, Dept Food Sci, I-80055 Naples, Italy

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 22期

关键词：

flow cytometry; friabilin; hardness; puroindolines; Real Time RT-PCR;

D O I：

10.1007/s10529-004-3745-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.

引用

页码：1731 / 1737

页数：7

共 6 条

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