The Arabidopsis thaliana cDNAs coding for eIF4E and eIF(iso)4E are not functionally equivalent for yeast complementation and are differentially expressed during plant development

被引：85

作者：

Rodriguez, CM ^{[1
]}

Freire, MA ^{[1
]}

Camilleri, C ^{[1
]}

Robaglia, C ^{[1
]}

机构：

[1] INRA, Biol Cellulaire Lab, F-78026 Versailles, France

来源：

PLANT JOURNAL | 1998年 / 13卷 / 04期

关键词：

D O I：

10.1046/j.1365-313X.1998.00047.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two cDNAs (At.EIF4E1 and At.EIF4E2) encoding, respectively, the eukaryotic initiation factors elF4E and elF(iso)4E of Arabidopsis thaliana were isolated by complementation of a Saccharomyces cerevisiae conditional mutant. The deduced amino acid sequences of the proteins are homologous to those from monocotyledonous plants, yeast and mammals. The corresponding genes were identified in YAC clones mapping to chromosome IV (At.EIF4E1) and to chromosome V (At.EIF4E2). The yeast strain complemented by At.EIF4E2 grew poorly compared with an isogenic strain expressing At.EIF4E1. Northern and in situ hybridization analysis show that both Arabidopsis At.EIF4E1 and At.EIF4E2 mRNAs are differentially accumulated in plant tissues. The At.EIF4E1 mRNA is expressed in all tissues except in the cells of the specialization zone of the roots; the At.EIF4E2 mRNA is particularly abundant in floral organs and in young developing tissues. This work further demonstrates an association between a high level of EIF4E mRNAs and cell proliferation and suggests that the plant elF4E isoforms may have distinct functions in cell development and metabolism.

引用

页码：465 / 473

页数：9

共 44 条

[11] The plant translational apparatus [J].

Browning, KS .

PLANT MOLECULAR BIOLOGY, 1996, 32 (1-2) :107-144

[12] A COMPARISON OF THE BINDING OF METHYLATED CAP ANALOGS TO WHEAT-GERM PROTEIN-SYNTHESIS INITIATION FACTOR-4F AND FACTOR-(ISO)4F [J].

CARBERRY, SE ;

DARZYNKIEWICZ, E ;

GOSS, DJ .

BIOCHEMISTRY, 1991, 30 (06) :1624-1627

[13] THE CIC LIBRARY - A LARGE INSERT YAC LIBRARY FOR GENOME MAPPING IN ARABIDOPSIS-THALIANA [J].

CREUSOT, F ;

FOUILLOUX, E ;

DRON, M ;

LAFLEURIEL, J ;

PICARD, G ;

BILLAULT, A ;

LEPASLIER, D ;

COHEN, D ;

CHABOUTE, ME ;

DURR, A ;

FLECK, J ;

GIGOT, C ;

CAMILLERI, C ;

BELLINI, C ;

CABOCHE, M ;

BOUCHEZ, D .

PLANT JOURNAL, 1995, 8 (05) :763-770

[14]

Dellaporta S.L., 1983, Plant Mol. Biol. Rep, V1, P19, DOI DOI 10.1007/BF02712670

[15] Control of root growth and development by cyclin expression [J].

Doerner, P ;

Jorgensen, JE ;

You, R ;

Steppuhn, J ;

Lamb, C .

NATURE, 1996, 380 (6574) :520-523

[16]

DONALDSON RW, 1991, J BIOL CHEM, V266, P3162

[17]

DUNCAN R, 1987, J BIOL CHEM, V262, P380

[18] PROTEIN-SYNTHESIS AND PROTEIN-PHOSPHORYLATION DURING HEAT-STRESS, RECOVERY, AND ADAPTATION [J].

DUNCAN, RF ;

HERSHEY, JWB .

JOURNAL OF CELL BIOLOGY, 1989, 109 (04) :1467-1481

[19] MODIFICATION OF EUKARYOTIC INITIATION-FACTOR 4F DURING INFECTION BY INFLUENZA-VIRUS [J].

FEIGENBLUM, D ;

SCHNEIDER, RJ .

JOURNAL OF VIROLOGY, 1993, 67 (06) :3027-3035

[20] THE HIGHER-PLANT ARABIDOPSIS-THALIANA ENCODES A FUNCTIONAL CDC48 HOMOLOG WHICH IS HIGHLY EXPRESSED IN DIVIDING AND EXPANDING CELLS [J].

FEILER, HS ;

DESPREZ, T ;

SANTONI, V ;

KRONENBERGER, J ;

CABOCHE, M ;

TRAAS, J .

EMBO JOURNAL, 1995, 14 (22) :5626-5637

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