The levels of integrin αvβ5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells

被引:56
作者
Takayama, K
Ueno, H [1 ]
Pei, XH
Nakanishi, Y
Yatsunami, J
Hara, N
机构
[1] Kyushu Univ, Sch Med, Chest Dis Res Inst, Fukuoka 8128582, Japan
[2] Kyushu Univ, Sch Med, Angiocardiol Res Inst, Mol Cardiol Unit, Fukuoka 8128582, Japan
[3] Kyushu Univ, Sch Med, Cardiovasc Clin, Fukuoka 8128582, Japan
关键词
adenovirus-mediated gene transfer; susceptibility; integrin; p53; lung cancer cells;
D O I
10.1038/sj.gt.3300608
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adenovirus-mediated transfer of growth-inhibiting molecules, such as p53, shows promise as an effective method of suppressing the growth of cancer cells. As the basis for in vivo studies, we examined transfection efficiency using 15 human lung cancer cell lines that differ in their endogenous p53 status. When infected with an adenovirus expressing bacterial beta-galactosidase, the different cell lines showed different levels of beta-galactosidase activity. We found a correlation between the level of integrin alpha(v) beta 5, which is thought to ben an adherence receptor for adenoviruses, and the expression level of the transferred gene, suggesting that gene expression is largely dependent on the infection efficiency. Growth inhibition was induced in all cell lines tested following infection with an adenovirus containing p53, provided a sufficient amount of p53 protein was expressed. Our results (1) confirm that the examination of the susceptibility of target cancer cells to an adenovirus is important when considering performing adenovirus-mediated gene transfer and for evaluating its therapeutic effects; and (2) suggest that the quantification of integrin alpha(v) beta 5 may be a good way of predicting the susceptibility of cells to adenoviral vectors.
引用
收藏
页码:361 / 368
页数:8
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