Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells

被引：69

作者：

Amatore, Christian ^{[1
]}

Arbault, Stephane ^{[1
]}

Chen, Yong ^{[1
]}

Crozatier, Cecile ^{[1
]}

Tapsoba, Issa ^{[1
]}

机构：

[1] Univ Paris 06, ENS, CNRS, UMR 8640 Pasteur, F-75231 Paris 05, France

来源：

LAB ON A CHIP | 2007年 / 7卷 / 02期

关键词：

D O I：

10.1039/b611569a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell.

引用

页码：233 / 238

页数：6

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