Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics

被引:43
作者
Erill, I
Jara, M
Salvador, N
Escribano, M
Campoy, S
Barbé, J
机构
[1] Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain
[2] Ctr Nacl Microelect, Biomed Applicat Grp, Bellaterra 08193, Spain
[3] CReSA, Bellaterra 08193, Spain
关键词
D O I
10.1093/nar/gkh996
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes (recA, ssb, uvrA and ruvCAB) might be a defining property of the Proteobacteria LexA network.
引用
收藏
页码:6617 / 6626
页数:10
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