CLN3 protein regulates lysosomal pH and alters intracellular processing of Alzheimer's amyloid-β protein precursor and cathepsin D in human cells

被引:91
作者
Golabek, AA [1 ]
Kida, E [1 ]
Walus, M [1 ]
Kaczmarski, W [1 ]
Michalewski, M [1 ]
Wisniewski, KE [1 ]
机构
[1] NYS Inst Basic Res, Dept Pathol Neurobiol, Staten Isl, NY 10301 USA
关键词
neuronal ceroid lipofuscinoses; CLN3; protein; APP; cathepsin D; lysosomal pH;
D O I
10.1006/mgme.2000.3006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Maintenance of the appropriate pH in the intracellular vacuolar compartments is essential for normal cell function. Here, we report that CLN3 protein, which is associated with the juvenile form of neuronal ceroid lipofuscinosis (JNCL), participates in lysosomal pH homeostasis in human cells. We show that CLN3 protein increases lysosomal pH in cultured human embryonal kidney cells, whereas inhibition of CLN3 protein synthesis by antisense approach acidifies lysosomal compartments. These changes in lysosomal pH are sufficient to exert a significant biological effect and modify intracellular processing of amyloid-beta protein precursor and cathepsin D, model proteins whose metabolism is influenced by the pH of acidic organelles. Mutant CLN3 protein (R334C) that is associated with the classical JNCL phenotype was devoid of biological activities of wild-type CLN3 protein. These data suggest that the pathogenesis of juvenile neuronal ceroid lipofuscinosis is associated with altered acidification of lysosamal compartments. Furthermore, our study indicates that CLN3 protein affects metabolism of proteins essential for cell functions, such as amyloid-P protein precursor, implicated in Alzheimer's disease pathogenesis. (C) 2000 Academic Press.
引用
收藏
页码:203 / 213
页数:11
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