In vitro plant regeneration from callus of shoot apices in apple shoot culture

被引：33

作者：

Caboni, E ^{[1
]}

Lauri, P ^{[1
]}

D'Angeli, S ^{[1
]}

机构：

[1] Ist Sperimentale Frutticoltura, I-00040 Rome, Italy

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 08期

关键词：

vegetative apex; callus; Malus domestica Borkh; shoot regeneration; tissue culture;

D O I：

10.1007/s002999900189

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A new reliable protocol for the induction of adventitious shoot formation and plant regeneration from apple callus has been developed. High regeneration frequency was obtained with this method in four different genotypes (Jork9, M26, Gala and McIntosh) and callus maintained regeneration ability for several months. The procedure consists of inducing vegetative shoot apices, excised from in vitro shoots, for 20 days in darkness on an MS medium without glycine, supplied with 17.8 mu M BA, 2.7 mu M NAA and 250 mg l(-1) cefotaxime. The explants are then transferred to a fresh auxin-free medium and given light. Histological studies revealed that all the regenerated shoots originated from callus. Regenerated shoots were multiplied, rooted and successfully established in soil.

引用

页码：755 / 760

页数：6