miR-146b is Highly Expressed in Adult Papillary Thyroid Carcinomas with High Risk Features Including Extrathyroidal Invasion and the BRAFV600E Mutation

被引:184
作者
Chou, Chen-Kai [1 ,2 ]
Chen, Rong-Fu [3 ]
Chou, Fong-Fu [4 ]
Chang, Hsueh-Wen [5 ]
Chen, Yi-Ju [6 ]
Lee, Ya-Fang [1 ]
Yang, Kuender D. [3 ]
Cheng, Jiin-Tsuey [5 ]
Huang, Chao-Cheng [6 ]
Liu, Rue-Tsuan [1 ]
机构
[1] Chang Gung Univ, Chang Gung Mem Hosp, Kaohsiung Med Ctr, Dept Internal Med,Div Metab, Kaohsiung, Taiwan
[2] Chang Gung Univ, Grad Inst Clin Med Sci, Kaohsiung, Taiwan
[3] Chang Gung Univ, Chang Gung Mem Hosp, Kaohsiung Med Ctr, Dept Med Res, Kaohsiung, Taiwan
[4] Chang Gung Univ, Chang Gung Mem Hosp, Kaohsiung Med Ctr, Dept Surg, Kaohsiung, Taiwan
[5] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 80424, Taiwan
[6] Chang Gung Univ, Chang Gung Mem Hosp, Kaohsiung Med Ctr, Dept Pathol, Kaohsiung, Taiwan
关键词
FACTOR-KAPPA-B; MICRORNAS; CANCER; DEREGULATION; ACTIVATION; IMMUNITY; PATHWAY; PLAYERS;
D O I
10.1089/thy.2009.0027
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
Background: Papillary thyroid carcinoma (PTC) are clinicopathogenetically heterogeneous. Micro-RNAs (miRNAs) are involved in the pathogenesis of diverse human cancers, including PTC. Information regarding associations between clinicopathological features of PTC with the expression of specific miRNAs, however, is sparse. In this study, we compared expression of deregulated miRNAs in PTCs to assess this was associated with selected clinicopathogenetic features. Methods: We analyzed the expression levels of three reported deregulated miRNAs (miR-221, miR-222, and miR-146b) using quantitative real-time polymerase chain reaction in 100 cases of PTCs with distinct clinicopathogenetic characteristics and 16 paired normal controls. The tumor samples were categorized into low- and high-risk groups on the basis of the tumor-node-metastasis staging system. Results: The expression levels of miR-221, miR-222, and miR-146b were significantly associated with extra-thyroidal invasion (p = 0.013, 0.05, and 0.003, respectively). The expression levels of miR-221 and miR-146b were significantly higher in the high-risk PTC group (p = 0.01 and 0.042, respectively). The miR-146b expression levels in PTCs with BRAF mutation were significantly higher than those without this mutation (p < 0.0001). There were no other associations between the expression of these miRNAs and other clinicopathological parameters. Conclusions: Our results show the potential importance of miR-221, miR-222, and miR-146b in determining the aggressive properties of PTCs and highlight the need to identify the gene targets of these miRNAs.
引用
收藏
页码:489 / 494
页数:6
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