HPLC determination of tetracyclines in lamb muscle using an RP-C18 monolithic type column

被引：11

作者：

Castellari, M ^{[1
]}

García-Regueiro, JA ^{[1
]}

机构：

[1] Inst Food & Agr Res & Technol, Food Chem Unit, Girona, Spain

来源：

CHROMATOGRAPHIA | 2003年 / 58卷 / 11-12期

关键词：

column liquid chromatography; monolithic column; tetracyclines; lamb muscle;

D O I：

10.1365/s10337-003-0068-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four antibiotics, oxytetracycline (OTC), tetracycline (TCC), chlortetracycline (CTC) and doxycycline (DXC) were separated and quantified in lamb muscle extracts using a commercial RP-C-18 monolithic HPLC column and a DAD detector. Operating an isocratic flow of 2 mL min(-1) the HPLC separation of the four compounds was obtained within 6 min with a good peak symmetry and an acceptable separation factor (1.27) for the critical peak pair OTC-TCC. Values of the normalized numbers of theoretical plates (N cm(-1)) were comprised between 338-780 while the limits of quantitation (S/N = 10) were within 12-65 mug kg(-1).

引用

页码：789 / 792

页数：4

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