Combining SPOT synthesis and native peptide ligation to create large arrays of WW protein domains

被引：46

作者：

Toepert, F

Knaute, T

Guffler, S

Pirés, JR

Matzdorf, T

Oschkinat, H

Schneider-Mergener, J

机构：

[1] Humboldt Univ, Charite, Inst Med Immunol, D-10115 Berlin, Germany

[2] Forschungsinst Mol Pharmakol, D-13125 Berlin, Germany

[3] Jerini AG, D-10115 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 10期

关键词：

peptides; protein arrays; protein design; protein modifications;

D O I：

10.1002/anie.200390298

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

More than 10000 variants of a 38 mer WW protein domain were prepared and screened successively with 22 different dye-labeled peptide ligands (see picture). This approach has enabled more than 250000 binding experiments to be performed and has revealed comprehensive information regarding the sequence requirements for binding. Thus, WW variants with novel binding specificities were identified.

引用

收藏

页码：1136 / 1140

页数：5

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