14-3-3ζ is an effector of tau protein phosphorylation

被引:182
作者
Hashiguchi, M
Sobue, K
Paudel, HK
机构
[1] Sir Mortimer B Davis Jewish Hosp, Bloomfield Ctr Res Aging, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada
[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H3T 1E2, Canada
关键词
D O I
10.1074/jbc.M003738200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neurofibrillary tangles associated with Alzheimer's disease are composed mainly of paired helical filaments that are formed by the aggregation of abnormally phosphorylated microtubule-associated protein tau. 14-3-3, a highly conserved protein family that exists as seven isoforms and regulates diverse cellular processes is present in neurofibrillary tangles (Layfield, R., Fergusson, J., Aitken, k, Lowe, J., Landon, NI., Mayer, R. J. (1996) Neurosci. Lett. 209, 57-60). The role of 14-3-3 in Alzheimer's disease pathogenesis is not known. In this study, we found that the 14-3-3 zeta isoform is associated with tau in brain extract and profoundly stimulates cAMP-dependent protein kinase catalyzed in vitro phosphorylation on Ser(262)/Ser(356) located within the microtubule-binding region of tan. 14-3-3 zeta binds to both phosphorylated and nonphosphorylated tau, and the binding site is located within the microtubule-binding region of tan. From brain extract, 14-3-3 zeta co-purifies with microtubules, and tubulin blocks 14-3-3 zeta-tau binding. Among four 14-3-3 isoforms tested, beta and zeta but not gamma and epsilon associate with tau. Our data suggest that 14-3-3(zeta) is a tau protein effector and may be involved in the abnormal tau phosphorylation occurring during Alzheimer's disease ontogeny.
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页码:25247 / 25254
页数:8
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